摘 要：摘 要：经典的蛋白质组学研究方法包括IEF/SDS-PAGE双向电泳和质谱技术的联用，但由于IEF的一些不足，限制了其应用范围。对角线电泳是蛋白质组学研究中的一项特殊分离技术，由于其原理与IEF/SDS-PAGE不同，正逐渐成为蛋白质组学中电泳分离技术的重要补充，特别是在膜蛋白和蛋白质相互关系的研究中将起到重要作用。本文综述了对角线双向电泳技术的特点、发展和在蛋白质组学研究中的最新进展，比较了双向电泳和对角线电泳的优缺点，展望了对角线电泳在蛋白质组学研究中的应用前景。
关键词：蛋白质组学；双向电泳；对角线双向电泳
Abstract: Abstract: The classical approach in proteomic analysis couples IEF/SDS-PAGE with mass spectrum. However, the shortcomings of IEF restrict its widespread applications. Diagonal polyarcylalmide gel electrophoresis, a special protein separation technique, shows its valuable application in proteomic research, especially in membrane proteins and protein-protein interaction because of its different feature characteristics from IEF/SDS-PAGE. This paper summarizes the characters, development and the latest applications of diagonal polyarcylamide gel electrophoresis in proteomics and the comparison between IEF/SDS-PAGE and the diagonal gel electrophoresis.
Key words: proteomics; 2-DE; two-dimensional diagonal electrophoresis
