《生命科学》 2018, 30(8): 896-905

长读长测序技术在宏基因组学研究中的应用

孙　丹1，袁文亮2，彭司华1*

(1 上海海洋大学水产与生命学院发育生物学系，水产种质资源发掘与利用教育部重点实验室，农业部国家水生动物病原库，科学技术部海洋生物科学国际联合研究中心，上海201306；2 上海理工大学光电信息与计算机工程学院，上海 200093)

摘　要：摘　要：由于很多微生物无法单独分离培养，研究微生物群落整体的宏基因组学是目前揭示微生物多样性的重要方法。长读长测序技术可以覆盖重复序列和复杂结构，获得短读长无法检测的基因组信息。现着重介绍了两类长读长测序技术，即基于第三代测序技术的单分子长读长测序技术和基于片段相互联系的合成长读长测序技术，并进一步介绍了长读长测序技术在宏基因组学领域的应用。

Application of long-read sequencing technologies in metagenomics research

SUN Dan1， YUAN Wen-Liang2， PENG Si-Hua1*

(1 Department of Developmental Biology of College of Fisheries and Life Science， Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources of Ministry of Education， National Pathogen Collection Center for Aquatic Animals of Ministry of Agriculture， International Research Center for Marine Biosciences of Ministry of Science and Technology， Shanghai Ocean University， Shanghai 201306， China; 2 School of Optical-Electric and Computer
    Engineering， University of Shanghai for Science and Technology， Shanghai 200093， China)

Abstract: Abstract: Because most microorganisms cannot be isolated and cultured alone， the study of the metagenomics of microbial communities is important to reveal microbial diversity. Long-read sequencing technology may cover repetitive sequences and complex structures， obtaining genomic information that cannot be detected by short-read sequencing. This review focuses on two types of long-read sequencing technology: single-molecule long-read sequencing technology based on third generation sequencing technology and synthetic long-read sequencing technology based on interconnection of DNA fragment. Furthermore， the application of long-read sequencing technology in metagenomics is also reviewed.