摘 要：摘 要：生物发光和荧光成像作为近年来新兴的活体动物体内光学成像技术，以其操作简便及直观性成为研究小动物活体成像的一种理想方法，在生命科学研究中得以不断发展。利用这种成像技术，可以直接实时观察标记的基因及细胞在活体动物体内的活动及反应。利用光学标记的转基因动物模型可以研究疾病的发生发展过程，进行药物研究及筛选等。本文综述了现有活体动物体内光学成像技术的原理、应用领域及发展前景，比较了生物发光与几种荧光技术的不同特点和应用。
关键词：活体动物体内光学成像；体内成像技术；生物发光；荧光素酶；荧光成像；多谱段成像；时域光学分子成像
Abstract: Abstract: Bioluminescence and fluorescence have been proved to be a versatile tool for in vivo imaging. Reporters that confer optical signals on a given biological process have been used widely in cell biology and more recently to interrogate biological process in animal models of human biology and disease. The theories, technologies, and the applications of these technologies will be compared and reviewed, especially the advantages and disadvantages between bioluminescence and fluorescence in vivo imaging. We also discussed the different technologies and applications about multi-spectral imaging and time-domain optical imaging, and reviewed the trend for future developments of these technologies.
Key words: optical in vivo imaging; animal imaging; bioluminescence; luciferase; fluorescence; multi-spectral imaging; time domain optical imaging
