《生命科学》 2018, 30(6): 628-636

细胞条件性重编程技术的研究进展

李　娜1，李　翔2，李一佳2*，陆彩霞1*

(1 中国医学科学院/北京协和医学院医学生物学研究所树鼩种质资源中心，昆明 650118；2 云南舜喜再生医学工程有限公司，昆明 650000)

摘　要：摘　要：细胞永生化培养技术一直是个难点，条件性重编程技术(conditional reprogramming， CR) 可能解决这个问题。CR 技术通过将铯源γ 射线辐照过的Swiss-3T3-J2 小鼠成纤维细胞和人正常细胞或肿瘤细胞共培养，向共培养体系中加入Rho 相关蛋白激酶(Rho-associated kinase， ROCK) 抑制剂Y-27632，使人正常细胞或肿瘤细胞获得部分干细胞特性和在体外无限制扩增的能力，并且撤除J2 细胞和ROCK 抑制剂之后，细胞能够重新定向分化为原细胞。CR 技术在再生医学、药物敏感性测试、基因表达谱分析和异种移植研究等领域具有广阔的发展前景，但目前尚不清楚其具体机制。现从CR 技术的研究现状、可能机制和实验方法进行综述，以期为CR 技术的研究和应用提供更多的理论基础。

Research progress of cell conditional reprogramming technology

LI Na1， LI Xiang2， LI Yi-Jia2*， LU Cai-Xia1*

(1 Center of Tree Shrew Germplasm Resources， Institute of Medical Biology，
    The Chinese Academy of Medical Sciences and Peking Union Medical College， Kunming 650118， China; 2 Yunnan Suns Regenerative Medicine Engineering Co.， Kunming 650000， China)

Abstract: Abstract: Cell immortalization has been difficult. Conditional reprogramming (CR) technology might be the solution. CR technology enables normal human cells and tumor cells to gain partial stem cell characteristics and proliferate indefinitely in vitro when co-cultured with irradiated Swiss-3T3-J2 mouse fibroblast feeder cells in the presence of Rho kinase inhibitor Y-27632. When cells were placed in conditions which mimic their natural environment， they retained the capacity to fully differentiate. CR cells have various applications including in regenerative medicine， drug sensitivity testing， gene expression profiling and xenograft studies. However， mechanisms of CR mediated immortalization are still unclear. This article summarized current research progress on CR mechanisms and experimental methods to provide theoretical evidences for future research and application.