《生命科学》 2017, 29(4): 349-355

B-LCLs及其在单克隆抗体生产中的应用

刘　玺1，2，3，陈新文1*，段子渊2*

(1 中国科学院武汉病毒研究所分子病毒学研究中心，武汉 430071；2 中国科学院遗传与
    发育生物学研究所遗传资源研究中心，北京 100101；3 中国科学院大学，北京 100049)

摘　要：摘　要：B 淋巴母细胞系(B-LCLs) 体外可以无限增殖，为很多实验持续提供基因型和表型匹配的原材料，极大地促进了生物医学研究的发展。近年来，B-LCLs 在单克隆抗体的生产、人类全基因组分析等方面得到重要应用，成为遗传进化研究的重要资源。现对B 淋巴母细胞系及其在单克隆抗体生产中的应用作一简述。

B-LCLs and its application in the production of monoclonal antibody

LIU Xi1，2，3， CHEN Xin-Wen1*， DUAN Zi-Yuan2*

(1 Molecular Virology Research Center， Wuhan Institute of Virology， Chinese Academy of Sciences， Wuhan 430071， China; 2 Genetic Resource Research Center， Institute of Genetics and Developmental Biology， Chinese Academy of Sciences， Beijing 100190， China; 3 University of Chinese Academy of Sciences， Beijing 100049， China)

Abstract: Abstract: B lymphoblastoid cell lines (B-LCLs)， which can proliferate indefinitely in vitro， fulfil the requirement of constant supply of starting material with matching genotypes and phenotypes for variety of assays， substantially accelerating the process of biomedical investigations. In recent years， its utility for producing monoclonal antibody and analyzing the whole human genome has extensively been documented. B-LCLs are increasingly being considered as an important resource for genetic and functional research. This article reviews the B-LCLs and its application in the production of monoclonal antibody.