摘 要：摘 要：依赖于NAD+的去乙酰化酶Sirtuin对细胞的存活、衰老、凋亡等生理活动的调节起到十分重要的作用。Sirtuin系统中的ySir2和SIRT1就目前来说是研究得较为透彻的两个成员。ySir2参与了酵母的交配型基因沉默、端粒的沉默、rDNA重复序列的沉默以及细胞寿命等生理功能。人类SIRT1在细胞存活与代谢等过程中也起到调节作用。本文对Sirtuin的结构、作用机制、底物特异性、影响因子及其功能作了综述。
关键词：组蛋白去乙酰化酶；NAD；沉默信息调节因子；SIRT1
Abstract: Abstract: The silent information regulator protein(Sir2) and its homologs(collectively known as Sirtuins) ，NAD+-dependent deacetylase enzymes, play an important role in physiological regulation, such as the survival，aging and apoptosis of cell. ySir2 is a member of the Sirtuin family, and the studies show it acts on the silencing of mating-type loci, telomeric DNA regions, ribosomal DNA repetition sequence and cellular aging. Another member, human SIRT1 also plays a part in regulation of cell survival and metabolism. In this review, we summarize the structure of Sirtuin and its function on gene silencing, metabolism and aging , and also discuss its molecular mechanism, substrate specificity, influence factor.
Key words: histone deacetylase; NAD; silent information regulator; SIRT1
