《生命科学》 2016, 28(6): 623-629
摘 要:摘 要:细胞全能性是指在多细胞生物体中,细胞经分裂和分化后仍具有形成完整有机体的潜能或特性。与动物相比,离体植物细胞在一定激素配比的培养基上即可表现出全能性。植物细胞全能性是实现植物再生和植物组织培养的理论基础。microRNA 是植物中普遍存在的小分子非编码RNA,在植物的各项生命活动中发挥着重要作用。作为基因表达的重要调控因子,其在调控植物细胞全能性、细胞分化与再生过程中扮演着至关重要的角色。着重介绍已知的参与植物全能性的miRNA 以及它们对植物再生过程的调控作用。
Abstract: Abstract: Plant cells are totipotent and competent to regenerate from differentiated organs through somatic embryogenesis and de novo organogenesis. De novo organogenesis refers to the in vitro formation of shoots or roots from culture explants. It is well known that shoot regeneration is dependent on phytohormone perception, acquisition of organogenetic competence, cell division progression, stem cell commitment and organ initiation.microRNA (miRNAs) are 21-24 nt noncoding gene regulators involved in many aspects of plant development. Here,we summarize the role of miRNAs in the acquisition of plant cell totipotency and de novo organogenesis.