microRNA调控植物细胞全能性与再生
刘晔彤1,张天奇2,3,高 健2,3,王佳伟2*
(1 上海师范大学生命与环境科学学院,上海 200234; 2 中国科学院上海生命科学研究院植物生理生态研究所植物分子遗传国家重点实验室,上海 200032;3 中国科学院大学,北京 100049)

摘 要:摘 要:细胞全能性是指在多细胞生物体中,细胞经分裂和分化后仍具有形成完整有机体的潜能或特性。与动物相比,离体植物细胞在一定激素配比的培养基上即可表现出全能性。植物细胞全能性是实现植物再生和植物组织培养的理论基础。microRNA 是植物中普遍存在的小分子非编码RNA,在植物的各项生命活动中发挥着重要作用。作为基因表达的重要调控因子,其在调控植物细胞全能性、细胞分化与再生过程中扮演着至关重要的角色。着重介绍已知的参与植物全能性的miRNA 以及它们对植物再生过程的调控作用。

The role of microRNAs in plant cell totipotency and regeneration
LIU Ye-Tong1, ZHANG Tian-Qi2,3, GAO Jian2,3, WANG Jia-Wei2*
(1 College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China; 2 National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological
    Sciences, Shanghai 200032, China; 3 University of Chinese Academy of Sciences, Beijing 100049, China)

Abstract: Abstract: Plant cells are totipotent and competent to regenerate from differentiated organs through somatic embryogenesis and de novo organogenesis. De novo organogenesis refers to the in vitro formation of shoots or roots from culture explants. It is well known that shoot regeneration is dependent on phytohormone perception, acquisition of organogenetic competence, cell division progression, stem cell commitment and organ initiation.microRNA (miRNAs) are 21-24 nt noncoding gene regulators involved in many aspects of plant development. Here,we summarize the role of miRNAs in the acquisition of plant cell totipotency and de novo organogenesis.

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