《生命科学》 2016, 28(5): 615-621

长链非编码RNA在体动物研究进展

申　涛，吴向琴，郭玉珠，汪香婷*

(中国科学技术大学生命科学学院，中科院分子细胞科学卓越创新中心，中科院脑功能与脑疾病重点实验室，合肥 230026)

摘　要：摘　要：从一开始被怀疑为基因组的噪音到目前成为生物医学研究领域的宠儿，主要基于细胞水平的长链非编码RNA (long noncoding RNA， lncRNA) 研究在过去的10 年左右时间里得到了迅猛发展。随着近几年在体动物研究的发展，lncRNA 领域正在由其新生阶段进入下一个重要发展阶段。然而，由于lncRNA 的特殊性，使得lncRNA 在体动物研究难度依然很大，对实验策略，甚至目的基因的选择都有很强的技巧。现就lncRNA 在体动物研究现状和其中一些重要问题进行综述。

Recent progresses of long noncoding RNA in vivo study

SHEN Tao， WU Xiang-Qin， GUO Yu-Zhu， WANG Xiang-Ting*

(CAS Center for Excellence in Molecular Cell Science， Chinese Academy of Sciences Key Laboratory of Brain Function and Disease， School of Life Sciences， University of Science and Technology of China， Hefei 230026， China)

Abstract: Abstract: From initially being considered as some genome noise to currently as one of the most extensively investigated topics in biomedical science， the long noncoding RNA (lncRNA) field has obtained a rapid growth during the past ten years or so， mainly based on studies in cultured cells. With the recent progresses on lncRNA in vivo investigations， the lncRNA field is stepping into a new era from its infancy. However， there is still a big challenge to the lncRNA in vivo study field， due to the distinction between lncRNA and the protein-encoded genes. Well consideration and appropriate approach are needed when choosing a modification strategy， or if possible， when choosing a targeted gene. We will discuss the research progresses of lncRNA in vivo study and several important issues in this review.