《生命科学》 2016, 28(1): 124-128

福氏志贺菌O-抗原磷酸乙醇胺修饰研究进展

张凤兰，徐　潇，权亚茹，崔生辉*

(中国食品药品检定研究院，北京 100050)

摘　要：摘　要：福氏志贺菌是发展中国家引起痢疾的主要致病菌。福氏志贺菌O- 抗原除噬菌体介导的糖基化和( 或)乙酰化外，最近发现一种新的修饰方式磷酸乙醇胺修饰，其机制为由质粒携带的opt 基因编码磷酸乙醇胺转移酶在O- 抗原的鼠李糖II 或( 和) 鼠李糖III 上添加磷酸乙醇胺基团，从而形成血清型Xv、4av 和Yv福氏志贺菌，并表达MASF IV-1 抗原。人工转化携带opt 基因的质粒到无MASF IV-1 抗原表达的不同血清型福氏志贺菌中，转化菌株都能表达MASF IV-1 抗原。在某些血清型福氏志贺菌中，O- 抗原的磷酸乙醇胺修饰与糖基化、乙酰化修饰之间相互作用。现对福氏志贺菌的O- 抗原磷酸乙醇胺修饰机制及其与糖基化、乙酰化修饰间的相互作用进行了综述。

Progress of O-antigen phosphoethanolamine modification of Shigella flexneri

ZHANG Feng-Lan， XU Xiao， QUAN Ya-Ru， CUI Sheng-Hui*

(National Institutes for Food and Drug Control， Beijing 100050， China)

Abstract: Abstract: Shigella flexneri is the major pathogen causing bacillary dysentery in the developing countries. Besides phage-encoded glucosylation and/or O-acetylation， O-antigen of S. flexneri modification with phosphoethanolamine (PEtN) has been identified lately. The plasmid-carried opt gene encodes the phospholethanolamine transferase adding a PEtN on O-antigen rhammose II and/or rhammose III. O-antigen PEtN modification of serotype X， 4a and Y strains convert the hosts into MASF IV-1 positive “variant” Xv， 4av and Yv serotypes respectively. The plasmidcarried opt gene is able to transform all S. flexneri serotypes to make them express MASF IV-1 epitope. Among various serotypes， the PEtN modification of O-antigen may interact with glucosylation and O-acetylation process. Now S. flexneri - O antigen ethanolamine phosphate modification mechanism and its interaction with glycosylation， acetylated modification were reviewed.