《生命科学》 2015, 27(10): 1306-1311

单细胞转录组测序在肿瘤异质性研究及临床中的应用

熊　虎1，张　蒙2，杨　立1*，吴　松2，3*

(1 兰州大学第二医院泌尿外科，兰州大学泌尿外科研究所，甘肃省泌尿系统疾病临床医学中心，甘肃省泌尿系统疾病研究重点实验室，兰州 730030；2 安徽医科大学深圳市第二人民医院临床医学院，深圳 518000；3 深圳市罗湖区人民医院， 深圳 518000)

摘　要：摘　要：随着现代生物医学的发展，基因测序技术已经在肿瘤学研究的多个领域得到广泛应用，但对肿瘤整体进行测序往往会掩盖细胞之间的异质性。为了探索单个肿瘤细胞的行为特性，肿瘤细胞异质性已成为从事肿瘤方面研究的科研和临床专家目前共同关注的焦点。单细胞转录组测序能从单个细胞的水平揭示同一肿瘤内不同细胞之间的变异程度与相互联系。随着单细胞分离、二代测序技术的发展，单细胞转录组测序日趋成熟。未来在临床中，单细胞转录组测序技术将会在肿瘤的早期诊断、监测、临床预后以及个体化用药等方面得到越来越广泛的应用。

The applications of single-cell RNA-Seq in the studies of intratumor    heretogeneity and clinical medicine

XIONG Hu1， ZHANG Meng2， YANG Li1*， WU Song2，3*

(1 Institute of Urology of Lanzhou University， Department of Urology， the Second Hospital of Lanzhou University， Clinical Center of Diseases of Urological System in Gansu Province， Key Laboratory of Diseases of Urological System in Gansu Province， Lanzhou 730030， China; 2 Shenzhen Second People’s Hospital， Clinical Medicine College of Anhui Medical University， Shenzhen 518000， China; 3 Luohu District People's Hospital of Shenzhen City， Shenzhen 518000， China)

Abstract: Abstract: With the development of modern biomedicine， gene sequencing technology has been widely used in many fields of oncology research. But sequencing the entire tumor perhaps conceals the heterogeneity between cells. In order to explore the behavior of the individual cell， intratumor heterogeneity becomes researchers’ and clinical experts’ common concern at present. Single-cell RNA-Seq is aimed to reveal the interrelation and variation between cells within the identical tumor at the level of single-cell. With the development of single-cell isolation and the next-generation sequencing (NGS) technology， the single-cell sequencing become more and more mature. In the future， the single-cell transcriptome sequencing technology will become more widely applied in early diagnosis， monitoring， clinical prognosis and individualized therapy of tumors in clinical practice.