《生命科学》 2015, 27(1): 45-49

基因修饰大鼠模型的研究进展

许　凯1，2，李　伟1*，周　琪1*

(1 中国科学院动物研究所，北京 100101；2 安徽大学生命科学学院，合肥 230601)

摘　要：摘　要：相比小鼠，大鼠在生理水平和认知能力等方面与人类更加接近，而且体型更大更容易操作，因而是研究人类疾病的良好模型。然而，由于遗传修饰技术发展的滞后，基因修饰大鼠模型的应用远远落后于小鼠。近年来，随着大鼠胚胎干细胞和位点特异性核酸酶等技术的发展，大鼠的遗传修饰得了一系列重要的进展。对各种遗传修饰技术在大鼠中的应用进行综述，为制备基因修饰大鼠模型提供参考。

Advances in the development of genome-modified rat models

XU Kai1，2， LI Wei1*， ZHOU Qi1*

(1 Institute of Zoology， Chinese Academy of Sciences， Beijing 100101， China;
    2 School of Life Sciences， Anhui University， Hefei 230601， China)

Abstract: Abstract: Rats are more similar to humans than mice in many aspects such as physiology and cognition， and also have larger body sizes that are convenient for manipulation， providing an important model for researching human diseases. However， due to the lack of effective genome-editing tools， the application of genome-modified rat models lags far behind that of mice. Recently， technologies on rat embryonic stem cells and site-specific nucleases have been developed， which bring many important advancements to genome modifications in rats. Here we review the application of various kinds of technologies for rat genome medication， which may help for the generation of genome-modified rat models for disease studies.