《生命科学》 2014, 26(12): 1255-1265

超高分辨率显微镜推进纳米生物学研究

任煜轩^*，于　洋，王　艳

中国科学院上海生命科学研究院生物化学与细胞生物学研究所国家蛋白质科学中心·上海(筹)，上海 201210

摘　要：超高分辨率显微镜是近年来生命科学领域重要的研究手段之一。2014 年诺贝尔化学奖颁发给超高分辨率显微技术领域的三位科学家，以表彰他们在该领域所作出的杰出贡献。超高分辨率技术的典型代表有受激损耗、结构光照明以及单分子定位等。这些技术的出现使得传统光学显微镜难以分辨的细胞器、分子等细节信息可以被观察到，帮助科学家从纳米尺度认识细胞内分子结构、定位以及相互作用。

Super-resolution microscopy in Nanobiology

REN Yu-Xuan^*， YU Yang， WANG Yan

National Center for Protein Sciences Shanghai， Institute of Biochemistry and Cell Biology，Shanghai Institutes for Biological Sciences， Chinese Academy of Sciences， Shanghai 201210， China

Abstract: Super-resolution microscopy is one of the advanced means for the study of life sciences. The 2014 Nobel prize in Chemistry was awarded to three scientists for their contributions to the super-resolution microscopy. Representative techniques achieving super-resolution are stimulated-emission， structured-illumination， and singlemolecule localization. The advancement of these techniques enable the visualization of the detail in cell organelle， macromolecules， and the localization of molecules. Such kind of information was unresolvable under traditional optical microscopes and will help understand the structure， location， interaction of molecules within cells in nanometer scale.