《生命科学》 2014, 26(2): 181-187

G蛋白偶联受体与G蛋白相互作用的最新研究进展

姜云璐1，2，龚　磊2，白　波2*，陈　京2，3*

(1 曲阜师范大学生命科学学院，曲阜 273165；2 济宁医学院神经生物学研究所，
    济宁 272000；3 英国华威大学华威医学院，考文垂 999020，英国)

摘　要：摘　要：传统观念认为，在激动剂作用下，G 蛋白偶联受体(GPCRs) 能够激活G 蛋白的α 亚基，从而使Gα 亚基与Gβγ 亚基分离，被激活的Gα 亚基通过信号转导进一步参与细胞的生理过程。但是，最新研究发现GPCRs 和G 蛋白存在多种偶联关系，GPCRs 不仅能够激活Gα 亚基，还可以与Gβγ 亚基相互靠近，甚至会使G 蛋白亚基构象发生重排而不分离，这对于疾病发病机制的研究及新的药物靶点的发现具有重要意义。就GPCRs 与G 蛋白之间的相互作用以及最新研究技术作一简要综述。

Advances in the research of interaction between G protein-coupled receptors and G proteins

JIANG Yun-Lu1，2， GONG Lei2， BAI Bo2*， CHEN Jing2，3*

(1 Department of Life Science， Qufu Normal University， Qufu 273165， China; 2 Department of Neurobiology， Jining Medical University， Jining 272000， China; 3 Warwick Medical School， University of Warwick， Coventry 999020， UK)

Abstract: Abstract: The interaction of G protein-coupled receptors (GPCRs) and G protein is an important part of signal transduction. Traditionally， GPCRs are able to activate Gα subunit following agonist stimulation， resulting in the dissociation of Gα subunit and Gβγ subunit. Activated Gα then participates in the physiological processes of cells. However， recent researches reveal there are multiple coupling relationships between GPCRs and G protein. GPCRs can interact with different subtypes of Gα and get close to Gβγ subunit. Even more， it was reported that activation of some heterotrimeric G proteins may trigger subunit rearrangement instead of dissociation of subunits， which is of great significance for researching the pathogenesis of disease and discovering new drug targets. In this paper， we review the interaction between GPCRs and G protein and the last research techniques.