摘 要：摘 要：在真核生物的分子生物学和遗传学研究方面，纤毛类原生动物嗜热四膜虫(Tetrahymena thermophila)已经被证明是一种有价值的生物学模型。通过对它的研究，人们发现并且掌握了核酶的分子机制、RNA的自我拼接、端粒的结构和功能、DNA序列重组等机理。这种原生动物的基因组功能分别由两个细胞核执行，即二倍体的小核与生殖过程有关，而多倍体的大核决定细胞的基因转录，并为转化基因的表达提供了强有力的手段。
关键词：嗜热四膜虫；功能基因组学；表达体系
Abstract: Abstract: Tetrahymena thermophila has been proven to be a valuable biological model for molecular and genetic studies of eukaryotic cells. Some investigations on the ciliated protozoan have provided the insights into the mechanism of ribozymes, self-splicing RNA, telomere structure and function, DNA sequence reorganization and so on. Compartmentalization of the genome into two functionally distinct nuclei, the silent micronucleus and the transcriptionally active macronucleus, provides a powerful means for controlling the expression of transgenes.
Key words: Tetrahymena thermophila; functional genomics; expression systems
