《生命科学》 2013, 25(11): 1135-1143

DNA拼接技术研究进展

张双华，孙　源，张治洲^*

(哈尔滨工业大学(威海)海洋科学与技术学院，山东省海洋船舶防污工程技术研究中心，威海 264209)

摘　要：摘　要：基因组合成是合成生物学的一个重要环节，其发展将会对未来的生物、医药、农业、能源等方面的发展产生巨大的推动作用，同时DNA 拼接作为基因组合成所需要的一种关键技术也日臻完备。回顾了多种DNA 拼接技术，并对其中最具发展潜力的几种方法作了综述，探讨不同DNA 拼接技术的原理和特点。

Research progress in DNA assembly technologies

ZHANG Shuang-Hua， SUN Yuan， ZHANG Zhi-Zhou^*

(School of Marine Science and Technology， Marine Antifouling Engineering Technology Center of Shangdong Province， Harbin Institute of Technology， Weihai 264209， China)

Abstract: Abstract: Genome synthesis constitutes an important part of synthetic biology， and its development will bring huge impact on many disciplines such as biology， medicine， pagriculture， energy and so on. By now DNA assembly is a key technology for genome or long DNA synthesis. Some DNA assembly technologies have acquired strong functional progress. In this review， a series of DNA assembly techniques are described and discussed in the context of their principles， potentials and characteristics.