《生命科学》 2013, 25(10): 983-992

合成生物学中的DNA组装技术

赵　鹃，王　霞，李炳志^*，程景胜，元英进

(天津大学化工学院系统生物工程教育部重点实验室，天津 300072)

摘　要：摘　要：合成生物学旨在应用工程学的研究思路及手段去设计或改造生物系统，是一个综合了科学与工程的拥有发展潜力的新兴学科，在生物医药、农业、能源、环保等方面发挥着巨大作用。DNA 组装技术是合成生物学中的关键技术，也是合成生物学快速发展的限制性技术。综述了众多DNA 组装技术的发展及其在合成生物学研究中的意义和应用。

DNA assembly in synthetic biology

ZHAO Juan， WANG Xia， LI Bing-Zhi^*， CHENG Jing-Sheng， YUAN Ying-Jin

(Key Laboratory of System Biological Engineering of the Ministry of Education，
    School of Chemical Engineering， Tianjin University， Tianjin 300072， China)

Abstract: Abstract: Synthetic biology aims to apply the engineering principles to the design of biological system. It is an emerging fi eld that combines science and engineering， and has great potentials in promoting the development in biomedical research， agriculture， bio-energy and bioremediation. DNA assembly is a pivotal technique implementing the vision of synthetic biology， and is also a limiting technology in advancing synthetic biology. This paper will review the development and current status of DNA assembly， and discuss its applications and significance
    in synthetic biology.