《生命科学》 2013, 25(10): 978-982

大肠杆菌最小基因组分析和删减进展

薛小莉^*，覃重军

(中国科学院上海生命科学研究院植物生理生态研究所，中科院合成生物学重点实验室，上海 200032)

摘　要：摘　要：大肠杆菌是基础研究最透彻、应用广泛的微生物，构建含减小甚至是最小基因组的大肠杆菌将为合成生物学的研究和应用提供理想的底盘生物。介绍了大肠杆菌最小基因组的生长与繁殖必需基因的生物信息学分析和实验鉴定，基因组敲除技术，以及删减基因组的大肠杆菌菌株的构建和应用等方面的研究进展。

Progresses in the analysis and development of Escherichia coli minimal genome

XUE Xiao-Li^*， QIN Zhong-Jun
    

(Key Laboratory of Synthetic Biology， Institute of Plant Physiology and Ecology，
    Shanghai Institutes for Biological Sciences， Chinese Academy of Sciences， Shanghai 200032， China)

Abstract: Abstract: Synthetic biology is a key research focus in recent years. Construction of Escherichia coli strains with reduced even minimal genome will provide a desired chassis cell for research work in synthetic biology. In this paper， progresses in the analysis and the identification of E. coli minimal genome and essential genes， the genome deletion techniques， and the construction and application of E. coli strains with reduced genome were reviewed.