《生命科学》 2013, 25(5): 532-538

植物miR164家族研究进展

牟桂萍，纪春艳，许东林，周国辉^*

(华南农业大学资源环境学院，广州 510642)

摘　要：摘　要： miRNA是真核细胞中一类长约22 nt的非编码小分子RNA，它们可通过碱基序列互补配对与靶mRNA分子结合并将后者降解，从而对靶基因实施转录后水平负调控，在生物发育调节及抗逆反应中发挥重要作用。miR164家族是植物特有的一类miRNA，其主要的靶基因是植物NAC家族转录因子。从miR164的特点、对NAC家族的调控、表达时序及组织分布特征和对环境及生物胁迫的响应，以及miR164及其靶基因的验证方法等方面，作一简要综述。
关键词：microRNA；miR164；基因调控；靶基因

Advances in plant miR164 family

MOU Gui-Ping， JI Chun-Yan， XU Dong-Lin， ZHOU Guo-Hui^*

(College of Natural Resources and Environment， South China Agricultural University， Guangzhou 510642， China)

Abstract: Abstract: MicroRNAs (miRNAs) are a class of non-coding small RNA molecules of ~22 nt in eukaryotic cells. They can complementarily combine with the mRNAs of target genes and cause their degradation， conducting post- transcriptionally negative regulation and thus playing important roles in a variety of biological growth/development processes and stress-resistance responses. The miR164 family is a group of plant-specific miRNAs， whose target genes mainly are the NAC transcriptional factor family. This paper presents a brief summary on the characteristics of the miR164 family， their regulation of NAC genes， their spatial-temporal expression and distribution and their responses to environmental or biological stress， as well as the methods of validation of miR164s and their target genes.
Key words: microRNA; miR164; gene regulation; target genes