《生命科学》 2012, 24(10): 1216-1222

复合式悬液芯片系统在病原学检测中的临床应用

侯　斐¹，王　红¹，罗　成²，王立魁^1*

(1 首都医科大学附属北京友谊医院，北京 100050；2 天津科技大学食品工程与生物技术学院，天津 300457)

摘　要：

 摘　要：复合式悬液芯片系统(multiplex suspension array system， MSAS)由悬浮芯片和微流体芯片组成，具有高通量精确定量的特点，而且具有较高的准确性和重复性，在多种蛋白质分子的同时精确定量和大规模样品检测方面有着很大的应用优势，其应用领域覆盖感染性疾病的快速诊断、细胞因子、信号通路、肿瘤标志物、流行病学病原体的筛查、蛋白质的相互作用、单核苷酸多态性(SNP)的研究等。就MSAS在病原学检测中的应用作一简要综述。

    

关键词：复合式悬液芯片系统；病原学检测；悬浮芯片技术

    

中图分类号：R446.5 文献标志码：A

Clinical application of multiplex suspension array system in pathogen detection

HOU Fei¹， WANG Hong¹， LUO Cheng²， WANG Li-Kui^1*

(1 Beijing Friendship Hospital Affiliated， Capital Medical University， Beijing 100050， China; 2 School of Food Engineering and Biotechnology， Tianjin University of Science and Technology， Tianjin 300457， China)

Abstract:

Abstract: Multiplex suspension array system (MSAS) is composed of suspension array and microfluidic chip， which demonstrates the features of high-throughput， precise quantification， accuracy， and repeatability. MSAS has great applicable advantages in accurate quantification for large-scale sample detection of various protein molecules， such as rapid diagnosis of infectious diseases， screening of cytokines， identification of signaling pathways， development of tumor markers and epidemiological pathogens. It has been used in the investigation of interaction proteins， single nucleotide polymorphism (SNP) analysis， etc. A briefly summary of the applications of MSAS in pathogen detection has been illustrated in this review.

    

Key words: multiplex suspension array system; pathogen detection; suspension array

    

收稿日期：2012-03-13； 修回日期：2012-06-26

    

基金项目：教育部留学回国人员科研启动基金[教外司留(2009)1001号]

    

*通信作者：E-mail: wanalbert69@gmail.com；Tel: 010-63138004