《生命科学》 2012, 24(10): 1082-1088

线粒体蛋白质组学研究进展

施苏雪¹，薛　凌¹，宋亚曼¹，卢　扬¹，韩俊刚¹，陈　红¹，卢中秋²，管敏鑫^1，3*

(1温州医学院Attardi 线粒体生物医学研究院，温州，325035；2温州医学院附属第一医院急救医疗室，温州，325000；3浙江大学生命科学学院，杭州，310058)

摘　要：

摘　要：线粒体是真核生物中重要的细胞器，其包含的全部蛋白质称为线粒体蛋白质组。人类线粒体大约包含1 500多种蛋白质，由核基因和线粒体基因共同编码。线粒体是细胞能量合成和物质代谢的中心，其功能障碍将直接或间接引起许多疾病。目前线粒体蛋白质组学正是系统性地研究线粒体在生理、病理过程中的功能变化以及研究疾病发生机制的重要方法。将线粒体蛋白质组的研究方法、研究进展、线粒体蛋白质组的性质及其在相关疾病研究中的作用进行综述， 并对线粒体蛋白质组学在疾病发生机制和诊断治疗中的发展前景进行展望。

    

关键词：线粒体；蛋白质组；呼吸链；质谱技术

    

中图分类号： Q51；R329.26　　文献标志码：A

Progress in the mitochondrial proteomics study

SHI Su-Xue¹， XUE Ling¹， SONG Ya-Man¹， LU Yang¹， HAN Jun-Gang¹， CHEN Hong¹， LU Zhong-Qiu²， GUAN Min-Xin^{1， 3*}

(1 Attardi Institute of Mitochondrial Biomedicine of Wenzhou Medical College， Wenzhou325035， China; 2 Emergency Medical Department， The First Affiliated Hospital of Wenzhou Medical College， Wenzhou 325000， China; 3 College of Life Science of Zhejiang University ， Hangzhou 310058， China)

Abstract:

    

    

Abstract: Mitochondria are important organelles in eukaryotes， entire proteins in the mitochondria are called mitochondrial proteome. Recently human mitochondrial proteome was identifified that consisted of 1500 proteins which were encoded by nuclear and mitochondrial DNA. Mitochondria are central to cellular energetic metabolism and material metabolism， while mitochondrial dysfunction will directly or indirectly leads to large numbers of diseases. However the mitochondrial proteome systematically analyses of the organelle in physiology and pathogenesis， also discovers the genetic basis of diseases. In the review， we introduce the methods and recent progress in the fifield of mitochondrial proteome; then we elaborate properties of the mitochondrial proteome and explore future prospects and challenges for using the proteome for systems analysis of the mitochondria.

    

Key words: mitochondria; proteome; respiratory chain; mass spectra