《生命科学》 2012, 24(7): 705-711

高通量测序技术及其在表观遗传学上的应用

张际峰^{1， 2*}，王 洋²，汪成润¹，聂刘旺³

(1 淮南师范学院生命科学系， 淮南 232001; 2 复旦大学生命科学学院， 上海 200433;3 安徽师范大学生命科学学院， 芜湖 241000)

摘　要：摘　要：DNA 测序技术是现代生命科学研究的重要工具之一，而高通量测序技术在全基因组的研究中发挥着越来越重要的作用。简要回溯DNA 测序技术的产生与发展，着重从PCR 扩增测序和单分子测序两个方面全面描述了高通量测序中众多代表性的技术及直接测序技术，并从DNA 甲基化、组蛋白修饰、非编码RNA 调控等方面阐述了高通量测序技术在表观遗传学上的运用。
关键词：高通量测序；表观遗传学；DNA 甲基化；组蛋白修饰；非编码RNA

High-throughput sequencing technology and its application in epigenetics

ZHANG Ji-Feng^{1， 2*}， WANG Yang²， WANG Cheng-Run¹， NIE Liu-Wang³

(1 Department of Life Science， Huainan Normal University， Huainan 232001， China; 2 School of Life Science， Fudan University， Shanghai 200433， China; 3 College of Life Science， Anhui Normal University， Wuhu 241000， China)

Abstract: Abstract: The DNA sequencing technology is one of the most powerful tools in modern life science research， and the high-throughput sequencing technologies play a key role in genomic sequencing of different organisms. Here， we presented a brief review of the invention and development of the DNA sequencing technologies. Furthermore， we described the representative sequencing technologies and their instruments of high-throughput sequencing technologies， which could be divided into two categories， PCR-based sequencing and single molecule sequencing.
Then we also provided insights into their future directions. In addition， the availability of high-throughput sequencing technologies has recently allowed the survey of genome-wide epigenetic variation， such as DNA methylation， histone modification and noncoding RNAs， and we described some of these approaches briefly.
Key words: high-throughput sequencing; epigenetics; DNA methylation; histone modification; noncoding RNAs