《生命科学》 2012, 24(7): 611-613

基于蛋白质组学的糖尿病相关生物标记物发现

曾　嵘^*，顾培明，李荣霞，苏智端，吴家睿

(中国科学院上海生命科学研究院生物化学与细胞生物学研究所，中国科学院系统生物学重点实验室，上海200032)

摘　要：摘　要： 近年来，随着生活水平的提高和生活方式的转变，我国糖尿病的发病率呈快速上升趋势。然而，目前对糖尿病的发生发展机制仍缺乏系统了解。最近本课题组结合多种蛋白质组学技术对糖尿病的发生发展过程进行研究，发现在大鼠2 型糖尿病的发生发展过程中，肝脏线粒体的多个代谢通路蛋白质表达及其修饰发生改变。在人群研究中发现，单氨基酸多态性与肥胖以及糖尿病的发展密切相关。此外，还发现了一些潜在糖尿病相关生物标志物，如载脂蛋白C-I、Ficolin-3 等。这些研究有助于完善对糖尿病发病机制的认识，同时也为防治糖尿病提供新的思路与方向。
关键词：生物标记物；肝脏线粒体；单氨基酸多态性；糖尿病

Discovery of diabetes-associated biomarkers based on proteomic research

ZENG Rong*， GU Pei-Ming， LI Rong-Xia， SU Zhi-Duan， WU Jia-Rui

(Key Laboratory of Systems Biology， Institute of Biochemistry and Cell Biology， Shanghai Institutes for Biological Sciences， Chinese Academy of Sciences， Shanghai 200031， China)

Abstract: Abstract: In recent years， the alteration of lifestyle renders the rapidly increasing incidence of diabetes in China. However， the pathogenesis of diabetes is lack of systemic understanding. We applied proteomics technologies to indepth study of diabetes pathogenesis. The incidence and development of type 2 diabetes affect protein expression and modifications in various metabolic pathways of liver mitochondrion in rat model. We found that single aminoacid polymorphisms closely associated with the development of obesity and diabetes. In addition， some potential biomarkers including Apolipoprotein C-I and Ficolin-3 were uncovered by proteomic approaches. These studies deepened the understanding of the pathogenesis of diabetes， then provided new clues for the prevention and cure of
diabetes.
Key words: biomarker; liver mitochondrion; single amino-acid polymorphisms; diabetes