《生命科学》 2012, 24(2): 169-173

L-谷氨酸氧化酶的研究进展

毕春元^1*，李　玲²，李敬龙²

(1 山东省科学院生物研究所，山东省生物传感器重点实验室，济南 250014； 2 山东轻工业学院食品与生物工程学院，济南 250353)

摘　要：摘　要：L- 谷氨酸氧化酶(L-glutamate oxidase， GLOD) 是一种以FAD 为辅基的黄素蛋白酶类，可以专一性地氧化谷氨酸生成过氧化氢、氨和α- 酮戊二酸，广泛应用于食品、医药、发酵等领域。从谷氨酸氧化酶的微生物来源、酶学性质、发酵条件、分离纯化及分析应用等方面进行阐述，并对其研究前景进行展望。
关键词：谷氨酸氧化酶；酶学特性；表达条件；分离纯化；应用；研究前景

Research progress on L-glutamate oxidase

BI Chun-Yuan^1*， LI Ling²， LI Jing-Long²

(1 Key Laboratory for Biosensors of Shandong Province， Biology Institute of Shandong Academy of Sciences， Jinan 250014， China; 2 School of Food and Bioengineering， Shandong Institute of Light Industry， Jinan 250353， China)

Abstract: Abstract: L-glutamate oxidase (GLOD) is a flavin protease with FAD for cofactor， and specifically catalyzes the reaction that one mole of L-glutamic acid was converted to one mole of hydrogen peroxide， α-ketoglutaric acid and ammonia with the consumption of one mole of oxygen and water. It has been widely used in the area of food， medicine， fermentation， etc. In this overview， several research aspects of GLOD are summarized， including microbial sources， enzymatic properties， fermentation conditions， purification and analytical applications of the enzyme. In addition， the research prospects of the L-glutamate oxidase are proposed.
Key words: L-glutamate oxidase; enzymatic properties; express conditions; purification; application; research prospect