《生命科学》 2011, 23(11): 1114-1129

噬菌体phi29 DNA包装马达磷脂膜嵌合体在单分子检测及纳米医学领域的应用

耿　佳，郭培宣^*

(美国辛辛那提大学医学院/工程学院，纳米生物医学中心，辛辛那提 45267，美国)

摘　要：摘　要：生命系统包含了具有不同功能的纳米机器和高度有序的大分子结构。所有的双链线性DNA 病毒使用由ATP 驱动的纳米分子马达将其基因包装在蛋白质外壳内。噬菌体phi29 DNA 包装马达的核心组成部分连接器已被成功嵌入到脂双层中，极为稳定且可用于离子和DNA 转运的精确测量。它在包装DNA 时具有单向通行的阀门机制，同时其关闭和打开可由人工控制。这对于详细研究分子马达的操作机制及未来医药应用中DNA 的包装、测序、采样和投递都具有重要意义。
关键词：纳米马达；连接器；离子通道；单通道电导；病毒组装；计量定量化；生物纳米技术；纳米生物技术

Membrane-embedded channel of bacteriophage phi29 DNA-packaging motor for single molecule sensing and nanomedicine

GENG Jia， GUO Pei-Xuan^*

(Nanobiomedical Center， College of Engineering and College of Medicine， University of Cincinnati， Cincinnati， OH 45267， USA)

Abstract: Abstract: The genome of linear double-stranded DNA (dsDNA) viruses is packaged into a preformed procapsid fueled by ATP hydrolysis. The central channel component of the bacteriophage phi29 motor is a connector protein， which has been successfully inserted into a lipid bilayer and exhibited robust capability for extremely reliable and precise assessment of the transportation of ions and DNA by single channel studies. The motor channel exercised a one-way traffic property for dsDNA translocation with a valve mechanism in DNA-packaging. In addition， the
opening and closure of the channel was also reversible and controllable. These findings have important implications since this artificial membrane-embedded channel would allow detailed investigations into discrete mechanisms of motor operation as well as future avenues for therapeutic dsDNA packaging， sampling， delivery and single molecule sensing.
Key words: nanomotor; connector; ion channel; single channel conductance; viral assembly; stoichiometry quantification; bionanotechnology; nanobiotechnology