《生命科学》 2011, 23(9): 882-890

微生物分解代谢物控制蛋白CcpA的研究进展

吴　艳，顾　阳，任　聪，杨　晟，姜卫红^*

(中国科学院上海生命科学研究院植物生理生态研究所，合成生物学重点实验室，上海 200032)

摘　要：摘　要：碳分解代谢物阻遏(carbon catabolite repression， CCR)是指微生物在混合碳源发酵时优先利用速效碳源(通常为葡萄糖)，且该碳源的代谢产物会抑制其他非速效碳源代谢相关的基因表达和蛋白活性，从而影响非速效碳源利用的现象。在低GC含量革兰氏阳性菌中，CCR效应的关键调控因子为分解代谢物控制蛋白CcpA(catabolite control protein A)。该调控蛋白具有多效性功能，除参与CCR外，还与中心碳、氮代谢的调控、生物被膜的形成和毒性基因的表达等多种生理过程相关。综述了近年来有关CcpA蛋白的功能、作用机制及分子结构的研究进展。
关键词：CcpA；碳分解代谢物阻遏效应；多效性；晶体结构

Recent research on catabolite control protein A in microorganisms

WU Yan， GU Yang， REN Cong， YANG Sheng， JIANG Wei-Hong^*

(Key Laboratory of Synthetic Biology， Institute of Plant Physiology and Ecology， Shanghai Institutes for Biological Sciences， Chinese Academy of Sciences， Shanghai 200032， China)

Abstract: Abstract: Carbon catabolite repression (CCR) is defined as the phenomenon that microorganisms preferentially utilize a rapidly metabolizable carbon source (normally glucose)， along with inhibition of some gene expression and enzyme activities related to catabolism of non-preferred carbon resources. In low-GC Gram-positive bacteria， the key regulator for exerting CCR is CcpA (catabolite control protein A)， which is a pleiotropic regulator involved in various physiological process in addition to CCR， including central carbon and nitrogen metabolism， biofilm formation and toxin gene expression. This paper reviewed the recent research advances of function mechanisms and molecular structure of CcpA. 
Key words: catabolite control protein A; carbon catabolite repression; pleiotropism; crystal structure