《生命科学》 2011, 23(9): 849-852

应用基因组工程构建新型大肠杆菌细胞工厂

蔡　钒^{1， 2}，方宏清^2*，陈必链¹

(1 福建师范大学生命科学学院，福州 350108；2 军事医学科学院生物工程研究所，北京 100071)

摘　要：摘　要：基因组工程(genome engineering)是指为了实现某一目标对复制系统进行有意地、广泛地遗传修饰。大肠杆菌作为常用细胞工厂之一，在生物制造领域应用广泛，已成为合成生物学的主要研究对象。应用基因组工程改造大肠杆菌可以进一步拓展其应用范围。介绍了基因组工程最新技术发展，如基因组整合、染色体进化、多元自动化基因组工程、可寻迹多元重组工程等，及其在改造大肠杆菌提高其生产能力、稳定性及环境耐受能力等方面的研究进展。
关键词：细胞工厂；大肠杆菌；基因组工程；合成生物学

Constructing novel E. coli cell factories by genome engineering

CAI Fan^{1， 2}， FANG Hong-Qing^2*， CHEN Bi-Lian¹

(1 College of Life Sciences， Fujian Normal University， Fuzhou 350108， China;2 Beijing Institute of Biotechnology， Beijing 100071， China)

Abstract: Abstract: Genome engineering is the extensive and intentional genetic modification of a replicating system for a specific purpose. Escherichia coli， one of the most common cell factories， is widely used in the domains of biological manufacturing and becomes a major subject in synthetic biology. Its application fields could be further expanded by genome engineering. This paper reviews the latest technology development of genome engineering， such as genome integration of heterogenous pathway， chromosomal evolution， multiplex automated genome engineering and trackable multiplex recombineering， and their application in improving the productivity， the stability and the tolerance of Escherichia coli as cell factories.
Key words: cell factories; Escherichia coli; genome engineering; synthetic biology