《生命科学》 2007, 19(2):

dsRNA介导植物基因沉默及其应用

曾晓珊^1,2，戴良英^1,3*，刘雄伦^1,2，王国梁^1,4*

1湖南农业大学水稻基因组学实验室，长沙410128；2湖南农业大学农学院，长沙410128；3湖南农业大学生物安全科技学院，长沙410128；4美国俄亥俄州立大学植物病理系，哥伦布 俄亥俄43210

摘　要：摘　要：植物双链RNA (double stranded RNA,dsRNA)能有效干扰同源基因的表达，近年来已成为功能基因组学研究上的新方法。本文综述了植物dsRNA介导的转基因沉默现象及其特点、分子作用机制、主要介导方法，以及近年来在植物功能基因组学研究上的应用情况。
关键词：RNA干扰; 转基因沉默; 双链RNA

Principles and applications of dsRNA- mediated gene silencing in plants

Zeng Xiaoshan^1,2, Dai Liangying^1,3*, Liu Xionglun^1,2, Wang Guoliang^1,4*

1The Rice Genomics Laboratory, Hunan Agricultural University, Changsha 410128,China; 2 The Agronomy College, Hunan Agricultural University, Changsha 410128,China; 3 The College of Bio-safety Science and Technology, Hunan Agricultural University, Chan

Abstract: Abstract: RNA interference(RNAi) is a homology-dependent gene silencing technology. The double-stranded RNA (dsRNA) can target the coding or promoter region of a gene directly. Using hairpin structures (hpRNA), double-stranded RNA can be expressed in plants relatively easily, enabling this technology to be applied to a wide range of species to silence the gene expression. In this review, we will discuss the principles, approaches and applications of the dsRNA-mediated gene silencing technique for plant gene functional analysis.
Key words: RNAi; gene silencing; dsRNA