《生命科学》 2011, 23(6): 605-611

N-糖基化位点鉴定方法和非经典N-糖基化序列

周　蕾，顾建新^*

(复旦大学上海医学院，卫生部糖复合物重点实验室，上海 200032)

摘　要：摘　要：蛋白质的N- 糖基化修饰是生物体调控蛋白质在组织和细胞中的定位、功能、活性、寿命和多样性的一种普遍的翻译后方式。N- 糖基化位点是理解糖链功能的重要前提之一。应用新的糖蛋白、糖肽富集技术和质谱技术，科学家们在不同组织中完成了对N- 糖基化位点的鉴定。此外，不同于经典三联子的N- 糖基化序列的发现使人们对N- 糖基化过程的认识向纵深发展。
关键词：N- 糖基化；糖基化位点；非经典序列
中图分类号：Q513.3 文献标志码：A

N-glycosylation sites analysis and nonconsensus N-glycosylated sequences

ZHOU Lei， GU Jian-Xin^*

(Key Laboratory of Glycoconjugate Research， Ministry of Health; Shanghai Medical College， Fudan university， Shanghai 200032， China)

Abstract: Abstract: N-glycosylation of proteins is the most common post-tranlational modification， strongly influencing many of their location， function， activity， life span and diversity. Our knowledge to N-glycosylation sites is one of the most important prerequisites for detailed functional understanding. Using new methods based on glycoproteins/glycopeptides enrichment and mass spectrum techniques， scientists have been able to identify glycosylated sites in different tissues. Besides， the discovery of novel sequence patterns for N-glycosylation will help to understand the process of N-glycosylation better in future.
Key words: N-glycosylation; glycosylation sites; nonconsensus sequences