《生命科学》 2011, 23(2): 212-215

基于微生物源的抗虫基因发掘

张四维，王　兵，王成树^*

(中国科学院上海生命科学研究院植物生理生态研究所，上海 200032)

摘　要：

摘　要：植物转基因抗虫技术在害虫控制方面取得了巨大成功。商业化运用的抗虫基因目前全部来源于苏云金杆菌(Bacillus thuringiensis， Bt) 的杀虫晶体蛋白基因，存在抗虫谱较窄及害虫逐渐产生抗性等问题，表明新型抗虫基因的筛选尤为重要。已有的文献研究表明，除了继续发掘Bt 来源的新型杀虫蛋白基因以外，非Bt 杀虫细菌及杀虫真菌也具有重要的发掘价值。

    

关键词：苏云金杆菌；非Bt 杀虫细菌；杀虫真菌；抗虫基因
中图分类号：S482. 292 ；Q786 文献标识码：A

Exploration of microbial origin insecticidal genes

ZHANG Si-Wei， WANG Bing， WANG Cheng-Shu^*

(Institute of Plant Physiology and Ecology， Shanghai Institutes for Biological Sciences， Chinese Academy of Sciences，Shanghai 200032， China)

Abstract:

Abstract: The technology of insect-resistant transgenic plants is being applied as a successful strategy for the control of insect pests. The insecticidal genes currently used in commercial genetically modified crops are all from the insect pathogenic bacterium Bacillus thuringiensis (Bt). After years’ applications， it is highly required to discover new genes to alleviate the issues of narrow anti-insect spectrum of Bt toxins and the occurrences of insect resistance against the toxic proteins. A review of the literatures shows that besides further studies of B. thuringiensis，
it is promising to explore novel microbial origin insecticidal genes from non-Bt insect pathogenic bacteria and insect-killing fungi.

    

Key words: Bacillus thuringiensis; non-Bt pathogenic bacteria; pathogenic fungi; insecticidal gene