《生命科学》 2010, 22(10): 985-990

自组装蛋白质芯片——功能蛋白质组学的新工具

艾润娜，赵晓航*

(中国医学科学院/北京协和医学院肿瘤研究所，分子肿瘤学国家重点实验室，北京100021)

摘　要：摘　要：传统的蛋白质芯片制备需要进行繁琐的蛋白质表达与纯化。同时，由于蛋白质活性不稳定，蛋白质芯片不宜长期保存。新一代自组装蛋白质芯片，利用无细胞表达体系和DNA固定技术，能够将蛋白质即时、原位表达并固定在芯片上，有效地解决了传统蛋白质芯片的制备和保存问题。目前自组装蛋白质芯片已初步用于大规模蛋白-蛋白质相互作用的筛选，以及鉴定免疫优势抗原等研究。该文介绍了近年自组装蛋白质芯片技术的进展和应用研究。
关键词：自组装蛋白质芯片；蛋白-蛋白质相互作用；无细胞蛋白质合成
中图分类号：Q816　　文献标识码：A

Self-assembling protein microarray—a new tool for functional proteomics

AI Run-na， ZHAO Xiao-hang*

(State Key Laboratory of Molecular Oncology， Cancer Institute， Chinese Academy of Medical Sciences & Peking Union Medical College， Beijing 100021， China)

Abstract: Abstract: Traditional protein microarrays require time-consuming processes of protein production and purification since the protein activities are not stable and the array products could not be stored properly for a long time. Self-assembling protein microarrays utilized cell-free expression system and DNA immobilization technique，  which allowed multiple-protein expression simultaneously and in situ immobilization on the surface of arrays. This technology effectively overcomes the problems of traditional protein microarrays， and provides a useful tool for functional proteomics analysis. Currently， self-assembling protein microarrays are primarily used for screening protein-protein interactions， identifying immunodominant antigens and so on. This review focused on the recent advancement and application research on self-assembling protein microarrays.
Key words: self-assembling protein microarray; protein-protein interaction; cell-free protein synthesis