《生命科学》 2010, 22(8): 743-748

人乳头状瘤病毒复制机制的研究进展

吕涛， 马正海*

（新疆大学生命科学与技术学院，新疆生物资源基因工程重点实验室，乌鲁木齐 830046）

摘　要：摘　要：人乳头状瘤病毒(human papillomavirus，HPV) DNA以游离和整合两种形式存在于感染细胞中。游离形式HPVs的复制依赖于上皮细胞的分化，病毒E1、E2蛋白和复制起始位点(origin，Ori)为复制必需元件，E1和E2蛋白与Ori结合起始病毒DNA的复制。随后，病毒DNA通过E2蛋白与Brd4 (bromodomain-containing protein 4)等细胞蛋白的互作而与染色体结合，并随细胞分裂平均分配到子代细胞中。在肿瘤中，高危型HPVs的基因组通常以整合形式存在，并随细胞的增殖而复制。
关键词：人乳头状瘤病毒；复制；E1；E2；复制起始位点

Research progress on replication mechanism of human papillomavirus

LV Tao， MA Zheng-hai*

(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering， College of Life Science and Technology， Xinjiang University， Urumchi 830046， China)

Abstract: Abstrac: Human papillomavirus DNAs exist in infected cells as episomal or integrated forms. The replication of episomal HPVs is closely linked to the differentiation of the host epithelial cells， and requires viral E1， E2， and origin (Ori). The binding of the E1 and E2 protein to the Ori triggers the initiation of replication， then the episomal viral genomes are tethered to mitotic chromosomes by E2 protein interaction with Brd4 or other cellular proteins， and segregated to daughter cells equitably. The viral DNAs of high-risk HPVs are often found integrated into the host chromosomes in tumor， and replicated along with the cells proliferation.
Key words: human papillomavirus; replication; E1; E2; replication origin