《生命科学》 2010, 22(3): 302-305

基因芯片技术在肿瘤缺氧微环境研究中的应用进展

刘　俊^*，赵　宁

(上海交通大学附属第一人民医院普外科，上海200080)

摘　要：

摘　要：缺氧微环境是实体肿瘤的特征之一，也是影响实体肿瘤发生、发展、侵袭、转移及耐药的重要因素，但其影响肿瘤的具体机制尚未完全明确。根据检测的mRNA数量，基因芯片分为传统的表达谱芯片和功能分类芯片。随着技术的发展，基因芯片技术因能对成千上万种基因的表达情况同时进行定量检测，在生命科学研究包括缺氧微环境对肿瘤作用的研究中越来越多地得到应用。选择性应用这些基因芯片除了能证明缺氧微环境与肿瘤发生发展有关外，同时还能筛选肿瘤缺氧微环境调节的未知靶基因及缺氧微环境重要调节因子的未知下游靶基因，还能为研究缺氧微环境影响肿瘤的分子机制提供有用的线索。基因芯片技术正成为该领域非常重要的研究工具之一。
关键词：基因芯片技术；缺氧微环境；肿瘤

The advances in the research on tumor hypoxia environment using DNA chip technique

LIU Jun^*， ZHAO Ning

(Department of General Surgery， Affiliated First People抯 Hospital， Shanghai Jiao Tong University， Shanghai 200080， China)

Abstract:

Abstract: Hypoxia environment is one of the characteristics of solid tumor， which affects the tumorigenesis， development， invasion， metastasis and chemo-resistance. But the mechanisms how the hypoxia environment affect tumor biological behavior are not yet fully clear. According to the number of mRNA detected， DNA chip technique can be classified to conventional expression profile chip and functional classification chip. DNA chip technique， which can simultaneously detect the expressions of thousands of genes， has been increasingly applied in life science research including the effect of hypoxia environment on tumor behaviors. With application of these gene chips， besides the association of hypoxic micro-environment with tumorigenesis， and tumor development was verified， the unknown target genes of hypoxic micro-environment and its important regulatory factors can be screened out， and useful clues to study the molecular mechanisms of the impact of hypoxia microenvironment on tumors are be  provided. DNA chip technique is becoming one of the essential tools in this field.
Key words: DNA chip technique; hypoxia environment; tumor