《生命科学》 2010, 22(2): 185-191

细菌锌离子抗性机制研究进展

季秀玲^*，魏云林，林连兵

（昆明理工大学生物工程技术研究中心，昆明 650224）

摘　要：

摘　要：锌(Zn)是生命代谢中重要的微量金属元素，但锌过量会对细胞造成毒害作用。细菌通过一些特有的机制来解除重金属离子对它们的毒害。该文重点介绍了细菌对高浓度Zn2+的抗性机制，主要包括外排机制(RND蛋白家族、CDF蛋白家族和P-型ATPase)、螯合机制和外排后结合机制。通过这些机制细菌能有效控制胞内Zn2+浓度，保护其不受过量Zn2+的毒害，但抗性机制往往不依赖单一的抗性系统，而是多种系统协调作用的结果。细菌Zn2+抗性机制的研究将有助于进一步揭示生物是如何应对高浓度金属离子的胁迫及相应的适应性规律。
关键词：外排机制；螯合；外排后结合；Zn2+

Research advances on zinc ion resistance mechanisms in bacteria

JI Xiu-ling^*， WEI Yun-lin， LIN Lian-bing

(Biotechnology Research Center， Kunming University of Science and Technology， Kunming 650224， China)

Abstract:

Abstract: Zinc， though an essential trace metal element， is toxic to the cell at higher concentrations. Mechanisms to evade metal ion toxicity are prevalent in bacteria. Mechanisms of zinc ion resistance are summaried in this paper. They range from efflux， sequestration， post-efflux binding to metal-responsive transcriptional regulatory proteins. Efflux-mediated zinc resistance mechanisms include RND system， CDF system and P-type ATPase. All these mechanisms aim at reducing the intracellular concentration of zinc ion so as to protect the cellular targets. zinc ion resistance is not unique function， but the result of multiple resistance systems. Mechanisms of zinc ion resistance are particularly of interest. It helps in the understanding of the homeostatic control of managing the excess amount of metal.
Key words: Efflux-mediated resistance mechanism; sequestration; post-efflux binding; Zn2+